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Extremely frustrating piece of software to support. We get calls from the same users over and over when trying to send something and getting the message that they need to the latest version but, it.
Due to Aspera license limitations, users have to install the software under their home to user the software.
- In the Preferences - Aspera Connect box that opens. Click Bandwidth; Set your Download Limit to: at least 10% less than your maximum available internet speed; Set your Upload Limit to: at least 10% less than your maximum available internet speed; Click OK; This will help avoid network saturation for other Internet access on your office network during Aspera Connect transfers.
- Before you can begin downloading data, you will need to install the Aspera Connect browser plugin. This is a free-of-charge piece of software which handles Aspera downloads either within a web browser - it works with the latest releases of all major browsers on Windows, Mac OS X, and Linux - or from the command-line.
Log on to O2
How to download all photos from iphone to macbook. If you need help connecting to O2, please review the Using Slurm Basic wiki page.
From Windows, use the graphical PuTTY program to connect to o2.hms.harvard.edu
and make sure the port is set to the default value of 22.
From a Mac Terminal, use the ssh
command, inserting your eCommons ID instead of user123:
Start interactive job, and create working folder
For example, for user abc123, the working directory will be
Download aspera and install it. You only need to do this once.
Use sratoolkit prefetch, which tries ascp then http, to download sra data, then convert the data from .sra to .fastq format
Additional tips:
- If you need download a lot of data, run screen command before starting interactive job, to keep the session alive:
screen: Keep Linux Sessions Alive (so you can go back to the same terminal window from anywhere, anytime) - If you a lot of samples to download, running prefetch command one by one is a lot of work. To automate the process, you can find the accession IDs from the website and put them in a loop to download one by one. For example to download SRR6519510 to SRR6519519:
for i in {6519510.6519519}; do
prefetch SRR$i;
done - If you have more than a dozens of samples to download, running one by one needs lot of time. You can run them in parallel, For example you submit 5 jobs, let each job work on 100 accession IDs. Because these 5 jobs share the same network from O2 to NCBI cloud, these parallel prefetch commands will run slower than in serial mode. Not sure how much slower. Please share your experience.
- Let us know if you have any questions.
IBM® Aspera® software supports upgrades against the two most recent versions of a release. If you submit a ticket for a product that precedes the two most recent versions, you’ll be referred to IBM professional services for upgrade assistance.
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